Question about PRF, the A nd the I PRF!!

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Posted on 12.07.2014 10:32 AM By Rafael Molina In Bio-modifiers: BMP-2 / PRGF

Good day Everyone on dental Xp excelente site!!
I am Rafael Molina from Bolivia, I am wanting to start using PRF and wanted to know if you with their experience in this can tell me if using a common centrifuge can get a good PRF?

Another question whether the PRF membrane holds about 5 months as a barrier between the gum and bone? substituting a membrane as bioguide? worth to use it?

I have my doubts and did not know who to ask more than here.

Thanks very much for your attention

Hug and greetings

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5 Comments

Maurice Salama says on 12.08.2014 07:09 AM

Rafael; Not sure about the Centrifuge and settings but PRF in the many different types requires different protocols for procurement. Also, it appears the Fibrins breakdown at 3-4 weeks. Good luck Dr. Salama

rocco mele says on 12.08.2014 09:30 AM

Dr Molina

peter smith says:
October 3, 2012 at 6:02 pm
I don’t know how I stumbled upon this thread. I am a periodontist with a research background. I just talked with Dr. Ziv Mazor over the weekend regarding another project and he agreed A centrifuge is a centrifuge. Angle has nothing to do with the way cells move around while being spun if a correct glass tube is being used. Any centrifuge from 2500- 3000rpm’s for 12 minutes will produce a nice fibrin clot. If a nice clot is not produced your centrifuge is off or you have a slightly contaminated tube. We use centrifuges that are $100-200 routinly and they work fine. best of luck

I saw this a while back. For what its worth ...
Rocco

sameh salama says on 12.10.2014 05:21 AM

Dr. Rocco
i have just posted another question above this thread and hoped for your involvement. i agree with most that a centrifuge is a centrifuge regardless but as i read from the papers everyone say from 2500-3000 rpm the speed. every centrifuge has a different distance from the central axis of its rotor to the end of the tube and hence affect the centrifugal force. dr. joseph suggested a force of 400 G and the PC02 centrifuge utilized "a hettich EBA model i presume" on 3000 rpm gives another g-force far apart from the 400 when i applied the {g = (1.118 × 10-5) R S2} formula
i have a table top centrifuge which gives me an optimal 400 G on 2000 rpm only. i used it and produced a very nice fibrin clot.
any suggestions ?

rocco mele says on 12.10.2014 09:34 AM

Dr Sameh
You have to remember I am treating animals of various sizes and the amount of blood taken is considerably less than you are getting from the human patient. So I had to develop a protocol ( trial and error ) of how much blood can be drawn safely, the ideal quantity to place in each red top tube, and how quickly it has to loaded and balanced in the centrifuge. If done properly we obtain really nice fibrin clots
Are all my measurements , angulations, and calculations correct... I don't know! Clinically, the results with soft tissue are especially encouraging.
If you are interested send me you e mail and i will forward some photos of my centrifuge and types of fibrin we are utilizing

Rocco

Jorge Campos says on 12.10.2014 09:47 AM

Hi Dr. Molina:
Any centrifuge can do the same work.
1500 rpm x 8 minutes work perfect!
Normal tubes.
I don´t think tha PRF last longer than 36 hs. It is only a concentrated fibrin preparation...so: it´s not a membrane for GBR. Only helps to improve healing and promotes activity of the cells thru growing factors.
Regards, Jorge.